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Table 3 Intracellular survival of P. multocida B:2 GDH7, P. multocida B:2 JRMT12 and P. multocida B:2 wild-type in BAEC at MOI 100:1

From: Interaction between Pasteurella multocida B:2 and its derivatives with bovine aortic endothelial cell (BAEC)

  No. of viable intracellular bacteria/BAEC
Bacterial strain Invasion time
(H)
Standard invasion assay No antibiotic added after washing P&G 10 μg/ml added after washing P&G 50 μg/ml added after washing
P. multocida B:2 GDH7 3 3.00 ± 0.13 - - -
5 - 2.62 ± 0.12 1.87 ± 0.17 1.90 ± 0.06
7 - 1.73 ± 0.04 1.61 ± 0.04 1.59 ± 0.05
P. multocida B:2 JRMT12 3 1.60 ± 0.09 - - -
5 - 0.93 ± 0.03 0.82 ± 0.06 0.67 ± 0.05
7 - 0.78 ± 0.03 0.75 ± 0.01 0.63 ± 0.01
P. multocida B:2 Wild type 3 1.22 ± 0.21 - - -
5 - 1.11 ± 0.04 0.94 ± 0.01 0.71 ± 0.04
7 - 0.93 ± 0.01 0.83 ± 0.01 0.78 ± 0.03
  1. Internalized viable bacteria were evaluated as no. of bacteria/BAEC after incubation of BAEC with bacteria for 2 h followed by exposure to polymyxin B and gentamicin (P&G) each at a final concentration of 50 μg/ml for 1 h prior to further incubation for 2 and 4 h in the presence of either 0, 10 or 50 μg/ml each of polymyxin B and gentamicin in the medium. Data represent the means (±SEM) of three independent assays with duplicate samples