Table 3 Intracellular survival of P. multocida B:2 GDH7, P. multocida B:2 JRMT12 and P. multocida B:2 wild-type in BAEC at MOI 100:1

Bacterial strain

Invasion time

(H)

Standard invasion assay

No antibiotic added after washing

P&G 10 μg/ml added after washing

P&G 50 μg/ml added after washing

P. multocida B:2 GDH7

3

3.00 ± 0.13

-

-

-

5

-

2.62 ± 0.12

1.87 ± 0.17

1.90 ± 0.06

7

-

1.73 ± 0.04

1.61 ± 0.04

1.59 ± 0.05

P. multocida B:2 JRMT12

3

1.60 ± 0.09

-

-

-

5

-

0.93 ± 0.03

0.82 ± 0.06

0.67 ± 0.05

7

-

0.78 ± 0.03

0.75 ± 0.01

0.63 ± 0.01

P. multocida B:2 Wild type

3

1.22 ± 0.21

-

-

-

5

-

1.11 ± 0.04

0.94 ± 0.01

0.71 ± 0.04

7

-

0.93 ± 0.01

0.83 ± 0.01

0.78 ± 0.03