Fig. 4

Localization of eukaryotic protein expression. Images showed BAEC with P. multocida B:2 GDH7 pSRGM at 5 h post-invasion. Cells were viewed under 60× objective lens with a Leica confocal laser scanning microscope. Images were taken of the same field under three different light paths; (I): Image taken under DIC (white) light. (II): Image taken under filtered fluorescent light (Exc. = 415 nm, Ems. = 493 nm) (blue) indicating the plasma membrane of BAEC. (III): Image taken under filtered fluorescent light (Exc. = 498 nm, Ems. = 540 nm) (green) indicating that the plasmid has been released into BAEC cytoplasm. (IV): Overlay of image (II) and 4.13 (III)