Fig. 5

H&E staining of the liver, kidney, brain, heart, lung, spleen from the rats used in the subchronic toxicity test. N = 3 per group, scale bar = 10 μm; 400×. a Representative section of heart tissue (Group I- VI), with no histological abnormality. b Liver, 0 g/kg, Group I, without histological abnormality. c Liver, Group VI animals, with expansion of liver sinusoids, d Liver, Group VI animals, with various degrees of vacuolar degeneration in hepatocytes. e Spleen, 0 g/kg (Group I), with no histological abnormality. f Spleen, Group VI rats, with decreased numbers of lymphocytes and increased numbers of macrophages. g Representative section of Lung, Group I-VI, with no histological abnormality. h Representative section of kidney, Group I-VI, with no histological abnormality. i Representative section of stomach, Group I-VI, with no histological abnormality. j Representative section of intestines, Group I-VI with not histological abnormality. k Representative section of brain, Group I-VI, with no histological abnormality. l Representative section of ovary, Group I- VI, with no histological abnormality. m Representative sections of testicle, Group I-V, with testicular tubules were arranged neatly and tightly. n Testicle, Group VI, with the number of sperm in seminiferous tubules appeared decreased