Fig. 4

Co-transfection with exogenous canine SGTA and REIC/Dkk-3 regulates AR signalling in human androgen-independent cancer cell line PC3. a Western blot analysis showing the expression of EGFP-tagged AR, Halo-tagged canine SGTA and HA-tagged canine REIC/Dkk-3 after co-transfection in a parallel experiment with that depicted in the graph b. b The effects of canine SGTA expression on the AR activity were examined in human prostate cancer PC3 cells under co-transfection with the AR, canine SGTA, and canine REIC/Dkk-3, with/without DHT. The results were obtained from three independent experiments, and show a significant difference in AR activity based on SGTA and/or REIC/Dkk-3 expression (*: P < 0.05, Student’s t-test). All transfection mixes were balanced with the appropriate empty vectors in terms of the ratio of the expression vectors and total plasmids. The value for the baseline was obtained using the control vehicle