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Fig. 3 | BMC Veterinary Research

Fig. 3

From: Canine REIC/Dkk-3 interacts with SGTA and restores androgen receptor signalling in androgen-independent prostate cancer cell lines

Fig. 3

Interaction between canine SGTA and REIC/Dkk-3 regulates AR signalling in canine androgen-independent prostate cancer cell line CHP-1. The upper graph shows the effects of canine REIC/Dkk-3 expression on AR signalling in the AR absent or present conditions with/without DHT stimulation in CHP-1 cells. Cells were co-transfected with the p159-pPr-Luc plasmid and treated with dihydrotestosterone (DHT). The value for the baseline was obtained using the control vehicle. The results were obtained from three independent experiments, and show a significant difference in AR activity based on REIC/Dkk-3 expression (*: P < 0.05, ANOVA). The lower figure shows the western blot results for the expression of EGFP-tagged AR, HA-tagged REIC/Dkk-3 and endogenous SGTA in CHP-1 cells after co-transfection in a parallel experiment with that depicted in the upper graph

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