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Fig. 2 | BMC Veterinary Research

Fig. 2

From: Canine REIC/Dkk-3 interacts with SGTA and restores androgen receptor signalling in androgen-independent prostate cancer cell lines

Fig. 2

Canine REIC/Dkk-3 interference in SGTA dimerization. The upper graph shows the role of canine REIC/Dkk-3, which interferes with SGTA dimerization, examined by a modified MTH assay. 293 T cells were co-transfected with DBD- or AD-fused canine SGTA plasmids and haemagglutinin (HA)-tagged REIC/Dkk-3. Luciferase activity in cell lysates was determined 48 h after transfection. The results were obtained from three independent experiments. The results show a significant difference between the SGTA alone and with the addition of 100 ng of canine REIC/Dkk-3 (*: P < 0.05, ANOVA). The lower panel depicts the western blot analysis showing the expression of DBD- and AD- fused canine SGTA, and haemagglutinin (HA)-tagged REIC/Dkk-3 after co-transfection in a parallel experiment with that depicted in the upper graph

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