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Fig. 1 | BMC Veterinary Research

Fig. 1

From: Canine REIC/Dkk-3 interacts with SGTA and restores androgen receptor signalling in androgen-independent prostate cancer cell lines

Fig. 1

Interaction between canine SGTA and REIC/Dkk-3. a Western blot analysis showing the expression profile of canine REIC/Dkk-3 and control β-actin in canine control fibroblasts and androgen-independent cell line CHP-1. b The interaction between canine SGTA and REIC/Dkk-3 was demonstrated by a mammalian two-hybrid (MTH) assay. DBD: GAL4-DNA-binding domain fusion protein. AD: VP16 transactivation domain fusion protein. The results were obtained from three independent experiments. c Western blot and pull-down assay results obtained from haemagglutinin (HA)-tagged REIC/Dkk-3 and Halo-tagged SGTA transfected 293 T cells lysates. d Co-localization of canine forced-expressed Halo-tagged REIC/Dkk-3 and endogenous SGTA was examined by double-immunofluorescence staining and observed by fluorescence microscopy. The images in green and red show the intracellular localization of Halo-tagged REIC/Dkk-3 and SGTA, respectively. The areas of overlap between REIC/Dkk-3 and SGTA are shown in yellow in the merged image

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BMC Veterinary Research

ISSN: 1746-6148

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