Potential for rapid antibody detection to identify tuberculous cattle with non-reactive tuberculin skin test results

Table 2 Diagnostic characterization of TST false-negative cattle identified in US herds infected with M. bovis

State	Animal ID #	CFT^a results		Postmortem examination results
State	Animal ID #	Jul-2015	Jan-2016	Gross lesions	Histopathology	PCR^b	Culture
TX	755	Pos	Neg	Present	TB compatible^c, IS6110 Pos^d	Neg	Neg
	272	Pos	Neg	Present	TB compatible	Pos	ND^e
	976	Neg	Neg	Present	TB compatible	Pos	ND
	857	Pos	Neg	Present	TB compatible	Pos	ND
	676	Pos	Neg	Present	TB compatible	Pos	ND
	889	Pos	Neg	Present	TB compatible	Pos	ND
	352	Neg	Neg	Present	TB compatible	Pos	ND
MI^f		Sep-2015	Jan-2016
	855	Neg	Neg	Present	TB compatible	ND	M. bovis
	370	Neg	Neg	Present	TB compatible	ND	M. bovis
	364	Neg	Neg	Present	Not compatible	ND	M. bovis
		May-2015	Oct-2015
	809	Neg	Neg	Present	TB compatible	ND	M. bovis

^aCFT, caudal fold test (i.e., intradermal M. bovis PPD injected into the caudal skin fold and response determined by palpation 72 h after injection) was applied <3 wks prior to collection of serum
^bPCR, polymerase chain reaction for either M. tb complex IS6110 or 1081 DNA on fresh tissue
^cMicroscopic granulomatous lesions consistent with bovine TB and containing acid-fast bacilli
^dIS6110 DNA by polymerase chain reaction on formalin-fixed tissue
^eND, not done
^fAnimals # 855, 370, and 364 were from a beef herd while #809 was from a dairy herd

ISSN: 1746-6148