Fig. 4

Copy number quantification of (a) IS1500 and (b) IS1533 by qRT-PCR in the Leptospira isolate and reference strain genomes. Bars represent the mean values of three replicates (±SD). IS copy number inference in other Leptospira spp. samples was performed using secY as the endogenous control and svr. Lai as the calibrator (asterisk)