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Fig. 2 | BMC Veterinary Research

Fig. 2

From: Molecular typing of Leptospira interrogans serovar Hardjo isolates from leptospirosis outbreaks in Brazilian livestock

Fig. 2

a PCR detection in Leptospira isolates and reference strains and (b) phylogenetic analysis of ORF22. The lanes in the gel (a) correspond to (1) molecular size marker, (2) svr. Lai, (3) svr. Copenhageni, (4) svr. Hardjo (OMS), (5) isolate Norma, (6) isolate Lagoa, (7) isolate Bolivia, (8) svr. Tarassovi, (9) svr. Mini, (10) svr. Hebdomadis, (11) svr. Sponselee and (12) negative control. The phylogenetic tree (b) was inferred using the neighbor-joining method (bootstrap: 1000, model: maximum composite likelihood) and rooted in the midpoint. The ORF22 sequences of the L. interrogans strains are separated into two clusters (gray rectangle). The L. kirschneri strains are separated into two clusters, and L. borgpetersenii is shown in one cluster. Samples representing our clinical isolates are indicated with arrows. Circles at nodes reflect the bootstrap support

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