Fig. 3

Circular cladogram representation of linear discriminant analysis effect size, LEfSe, of the 16S rRNA gene sequences. The sequences were obtained from faecal samples taken following different diet periods from 11 healthy, client-owned dogs during the seven-week dietary intervention study. A mean value of the three samples collected from each of the dogs in each of the diet periods were used for this analysis. The diet periods were as follows: CD1 for week 1 and 2, during which all dogs were acclimated to commercial dry food (CD; Felleskjøpet’s Labb adult), followed by incremental substitution of the CD diet with minced beef − LMB, low minced beef for week 3, MMB, moderate minced beef for week 4, and HMB, high minced beef for week 5 – and finally, CD2 for week 6 and 7, during which the dogs were reintroduced to the CD diet. The data points represent OTUs identified at phylum level in the centre of the circle (name not given), and genus level in the outer circle. The OTUs present in differential relative abundances in samples from the diet periods − red: CD1 and CD2, and green: HMB − are listed in the upper right corner. The yellow points indicate OTUs that are not present in differential relative abundances in samples from diet periods. Figure (a) depicts data from CD1 vs. HMB, and (b) depicts data from CD2 vs. HMB and (α = 0.05, LDA score > 2.0). P-value for CD1 vs. HMB was determined for 9 dogs and P-value for CD2 vs. HMB was determined for 8 dogs. No significant differences were detected between CD1 or CD2 vs. LMB or MMB and are therefore not included in the figures