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Table 6 Comparison of observed quality parameters for the Gentian Canine CRP Immunoassay run on three different analyzers

From: Evaluation of a species-specific C-reactive protein assay for the dog on the ABX Pentra 400 clinical chemistry analyzer

Quality parameters Gentian CRP

ABX Pentra 400

Abbott Architect c4000a

Olympus AU600b

Limit of quantification (mg/l)

4.0c

6,8d

5.4e

Linearity (mg/l)

4–281

6.8–1201

~5–100

Recovery (%)

90–105

116–123

105–118

No prozone effect (mg/l)

≤ 676

≤ 1200

≤ 400

Precision

CRP range (mg/l):

< 270; > 25

< 270; > 25

≤ 100, ≥ 50

Intra-assay CV (%)

0.7–1.9

0.5–1.7

1.0–1.3

Inter-assay CV (%)

   

- Between run CV (%)

0.9–2.1

0.0–0.3

n.d.

- Between day CV (%)

1.1–4.6

1.1–1.9

4.1–4.7

No Interference up to

- Hemoglobin (g/l)

5

10

n.d.

- Bilirubin (mg/l)

800

n.d.

n.d.

- Triglycerides (g/l)

10

10

n.d.

Method comparison

Reference method

Randox Canine CRP assay

Randox Canine CRP assay

Olympus CRP

- rs

0.98

0.995

0.96

- intercept

-1.18

7.3

n.d.

- slope

0.99

0.92

n.d.

- mean constant bias (%)

5.2

n.d.

41.0–70.5f

- TEobs (%) (~CRP 7–60 mg/l)

9.5–20.9

n.d.

50.4–82.6 f

(~CRP > 100 mg/l)

7.0–8.9

n.d.

n.d

Sample type comparison

small impact

n.d.

n.d.

  1. The assay was evaluated independently on the ABX Pentra 400 and compared to the data of the previous validation on the Abbott Architect c4000 and on the Olympus AU600.
  2. Abbreviations: n.d. not done, CV coefficient of variation, TE obs observed total allowable error
  3. a(Hillström et al. 2014) b(Muñoz-Prieto et al.2017)cbased on the desirable CV < 12.16%, dbased on desirable TE < 29.58%; both published in the addendum of the Total allowable error (TE) guidelines of the American College of Veterinary Clinical Pathology [32], ebased on CV < 20% (Escribano et al. 2012). f calculated based on the data of healthy and diseased dogs evaluated by Muñoz-Prieto et al.: healthy dogs: \( bias\%=\frac{median\ control- median\ test}{mean\ of\ the\ medians}=\frac{2.82\frac{mg}{l}-1.35\frac{mg}{l}}{\left(2.82-1.35\right):2} x100\%\approx 70.5\% \); TEobs = bias % + 2CV% = 70.5% + 2 × 6.05% = 82.6%
  4. dogs with inflammatory conditions:\( bias\%=\frac{73.7\frac{mg}{l}-48.6\frac{mg}{l}}{\left(73.7+48.6\right):2} x100\%\approx 41.0\% \);TEobs = 41.0% + 2 × 4.70% = 50.4%