Molecular characterization of hemotropic mycoplasmas (Mycoplasma ovis and ‘Candidatus Mycoplasma haemovis’) in sheep and goats in China

Table 1 Primers used for amplification of the 16S rRNA gene of hemoplasmas (Mycoplasma ovis and ‘Candidatus Mycoplasma haemovis’)

Primers	Nucleotide sequences	Target fragment/bp	Remarks
A1	5′-GGATAGCAGCCCGAAAGG-3′	1060	1st PCR
A2	5′-GCAGCCCAAGGCATAAGG-3′	1060	1st PCR
B1	5′-CTACGGGAAGCAGCAGTG-3′	506 or 489	Nested PCR [32]
B2	5′-CTCGACCTAACATCAAATACCT-3′	506 or 489	Nested PCR [32]
16S Fw	5′-ATGCAAGTCGAACGAGTAGA-3′	1341	Conventional PCR [26]
16S Rv	5′-TGATACTTTCTTTCATAGTTTG-3′	1341	Conventional PCR [26]

ISSN: 1746-6148