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Table 1 Primers used for amplification of the 16S rRNA gene of hemoplasmas (Mycoplasma ovis and ‘Candidatus Mycoplasma haemovis’)

From: Molecular characterization of hemotropic mycoplasmas (Mycoplasma ovis and ‘Candidatus Mycoplasma haemovis’) in sheep and goats in China

Primers Nucleotide sequences Target fragment/bp Remarks
A1 5′-GGATAGCAGCCCGAAAGG-3′ 1060 1st PCR
A2 5′-GCAGCCCAAGGCATAAGG-3′
B1 5′-CTACGGGAAGCAGCAGTG-3′ 506 or 489 Nested PCR [32]
B2 5′-CTCGACCTAACATCAAATACCT-3′
16S Fw 5′-ATGCAAGTCGAACGAGTAGA-3′ 1341 Conventional PCR [26]
16S Rv 5′-TGATACTTTCTTTCATAGTTTG-3′