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Table 3 Performance of the RespoCheck Mycoplasma real-time PCR in which the M. dispar, M. bovis and M. bovirhinis DNAs were diluted in PBS (A) or cells were spiked in BALF (B)

From: Mycoplasma detection by triplex real-time PCR in bronchoalveolar lavage fluid from bovine respiratory disease complex cases

A

PCR

Agent

Real time PCR

R2

Slope

Efficiency (%)

Linearity (ng)

Detection limit (ng/assay)

Singleplex PCR

M. dispar

0.9995

−3.3836

97.49

10 ng-10 fg

10 fg

M. bovis

0.9966

−3.1137

109.49

10 ng-10 fg

10 fg

M. bovirhinis

0.9955

−3.5033

92.95

10 ng-10 fg

10 fg

Triplex PCR

M. dispar

0.9989

−3.1175

109.3

10 ng-10 fg

10 fg

M. bovis

0.9955

−3.6240

88.8

10 ng-100 fg

100 fg

M. bovirhinis

0.9939

−3.3735

97.9

10 ng-100 fg

100 fg

B

PCR

Agent

Real time PCR

R2

Slope

Efficiency (%)

Linearity (CFU/ml; log 10)

Detection limit (CFU/assay)

Singleplex PCR

M. dispar

0.995

3.248 ± 0.1276

103.2

6.5–2.5

1–2

M. bovis

0.995

3.453 ± 0.1178

94.8

6.5–1.5

0.5

M. bovirhinis

0.981

3.395 ± 0.2698

97.0

5.5–1.5

0.5

Triplex PCR

M. dispar

1.000

3.534 ± 0.04608

91.9

6.5–2.5

1–2

M. bovis

0.993

3.462 ± 0.1440

94.5

6.5–1.5

0.5

M. bovirhinis

0.965

2.750 ± 0.3014

131.0

5.5–1.5

0.5