TY - JOUR AU - Cornelissen, Jan B. W. J. AU - de Bree, Freddy M. AU - van der Wal, Fimme J. AU - Kooi, Engbert A. AU - Koene, Miriam G. J. AU - Bossers, Alex AU - Smid, Bregtje AU - Antonis, Adriaan F. AU - Wisselink, Henk J. PY - 2017 DA - 2017/04/08 TI - Mycoplasma detection by triplex real-time PCR in bronchoalveolar lavage fluid from bovine respiratory disease complex cases JO - BMC Veterinary Research SP - 97 VL - 13 IS - 1 AB - In this study we evaluated the RespoCheck Mycoplasma triplex real-time PCR for the detection in bronchoalveolar lavage fluid (BALF) of Mycoplasma (M.) dispar, M. bovis and M. bovirhinis, all three associated with bovine respiratory disease (BRD). Primers and probes of the RespoCheck Mycoplasma triplex real-time PCR are based on the V3/V4 region of the 16S rRNA gene of the three Mycoplasma species. SN - 1746-6148 UR - https://doi.org/10.1186/s12917-017-1023-6 DO - 10.1186/s12917-017-1023-6 ID - Cornelissen2017 ER -