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Table 5 Changes in mean concentration of Estradiol hormone in prepubertal buffalo heifers following inoculation with Pasteurella multocida B: 2 and its immunogens (LPS and OMP)

From: Reproductive hormonal variations and adenohypophyseal lesions in pre-pubertal buffalo heifers inoculated with Pasteurella multocida type B: 2 and its immunogens

Time (hr)

Inoculated with phosphate buffer saline

Inoculated with P.multocida B:2

Inoculated with LPS extracted from P.multocida B:2

Inoculated with OMPs extracted from P.multocida B:2

Control –G1

Oral route -G2

Subcutaneous route - G3

Oral route- G4

Intravenous route – G5

Oral route - G 6

Subcutaneous route - G7

0

51.26 ± 0.56a

51.43 ± 0.00a

49.99 ± 0.45ab

48.91 ± 0.05b

50.72 ± 0.42a

49.19 ± 0.11ab

48.32 ± 0.58b

2

52.19 ± 0.02a

41.12 ± 0.01d

43.99 ± 0.01bc

43.57 ± 0.33bc

42.96 ± 0.02cd

41.13 ± 0.08d

44.32 ± 0.02b

4

48.16 ± 0.01a

40.65 ± 0.19cd

32.55 ± 0.51d

41.46 ± 0.01c

40.16 ± 0.58cd

40.46 ± 0.27cd

42.61 ± 0.03b

6

50.01 ± 0.00a

39.51 ± 0.00b

30.31 ± 0.18f

38.74 ± 0.15d

39.07 ± 0.00c

39.07 ± 0.00c

38.22 ± 0.052e

8

48.33 ± 0.58a

37.47 ± 0.64ab

27.76 ± 0.14d

36.18 ± 0.10b

38.11 ± 0.01ab

38.89 ± 0.51ab

35.24 ± 0.017c

10

48.11 ± 0.02a

36.21 ± 0.00c

26.66 ± 0.38f

34.98 ± 0.01d

37.76 ± 0.14b

35.31 ± 0.18d

29.7 ± 0.64e

12

50.31 ± 0.10a

32.57 ± 0.25c

20.33 ± 0.00e

34.72 ± 0.12bc

36.78 ± 0.13b

34.76 ± 0.44bc

29.85 ± 0.02d

24

51.22 ± 0.00a

31.19 ± 0.02c

 

33.91 ± 0.58b

32.84 ± 0.49b

30.06 ± 0.00d

28.34 ± 0.06e

36

48.41 ± 0.08a

30.88 ± 0.07c

 

33.02 ± 0.00b

30.35 ± 0.00cd

29.92 ± 0.53d

28.77 ± 0.13d

48

50.73 ± 0.00a

30.43 ± 0.58bc

 

31.33 ± 0.19b

30.12 ± 0.58bcd

27.18 ± 0.58d

27.99 ± 0.01cd

60

49.05 ± 0.58a

28.18 ± 0.02bc

 

30.93 ± 0.04b

28.17 ± 0.10bcd

26.14 ± 0.58cd

25.43 ± 0.10d

72

52.33 ± 0.06a

28.04 ± 0.00d

 

31.2 ± 0.5774c

36.78 ± 0.13b

24.41 ± 0.50e

22.17 ± 0.00f

120

49.13 ± 0.05a

27.59 ± 0.34c

 

28.37 ± 0.00b

26.34 ± 0.19d

23.96 ± 0.02e

 

168

52.19 ± 0.04a

26.78 ± 0.13b

 

26.66 ± 0.38b

24.03 ± 0.00b

22.19 ± 0.00c

 

216

51.64 ± 0.21a

26.33 ± 0.00b

 

26.31 ± 0.40bc

22.47 ± 0.31bc

22.35 ± 0.57c

 

264

48.16 ± 0.58a

26.42 ± 0.66b

 

25.93 ± 0.54bc

22.15 ± 0.02c

22.49 ± 0.38bc

 

312

48.41 ± 0.02a

24.98 ± 0.23b

 

24.06 ± 0.04c

21.14 ± 0.02c

22.01 ± 0.56c

 

360

48.1 ± 0.58a

24.09 ± 0.18b

 

23.54 ± 0.27bc

20.94 ± 0.26c

20.21 ± 0.58c

 

408

50.73 ± 0.16a

22.27 ± 0.15b

 

22.2 ± 0.318bc

20.91 ± 0.43cd

19.87 ± 0.50d

 

456

50.2 ± 0.058a

20.17 ± 0.10c

 

21.88 ± 0.29c

20.66 ± 0.25c

19.43 ± 0.00d

 

504

49.53 ± 0.38a

19.99 ± 0.01bc

 

21.09 ± 0.00b

20.03 ± 0.16bc

18.18 ± 0.10c

 
  1. All values are expressed as mean ± SE; a, b, c, d, e, f, g, values with superscript within rows are significantly different at P < 0.05