TY - JOUR AU - Nardini, Roberto AU - Autorino, Gian Luca AU - Issel, Charles J. AU - Cook, R. Frank AU - Ricci, Ida AU - Frontoso, Raffaele AU - Rosone, Francesca AU - Scicluna, Maria Teresa PY - 2017 DA - 2017/04/14 TI - Evaluation of six serological ELISA kits available in Italy as screening tests for equine infectious anaemia surveillance JO - BMC Veterinary Research SP - 105 VL - 13 IS - 1 AB - ELISAs are known to have a higher diagnostic sensitivity than the agar gel immunodiffusion (AGID) when employed for serological diagnosis of equine infectious anaemia (EIA). For this purpose, an “in-house” and five commercial ELISAs available in Italy were assessed by the National Reference Centre for EIA for their analytic specificity (Sp); precocity, defined as capability of detecting first antibodies produced during a new infection; precision based on repeatability and reproducibility, estimated from the coefficient of variation (CV); accuracy, estimated from multiple K and relative Sp and sensitivity (Se). Two serum panels, positive for non-equine retroviruses and the most frequent equine viruses, were employed to measure analytic Sp. ELISA precocity was also compared to that of one “in-house” and three commercial AGID kits, employing a panel of sera, collected weekly from horses infected with modified EIA viruses. Precision and accuracy were defined using results of a panel containing positive and negative sera examined in an inter-laboratory trial with the participation of the ten Official Laboratories. Furthermore, a questionnaire was used to assess the appropriateness of each kit for routine use. SN - 1746-6148 UR - https://doi.org/10.1186/s12917-017-1007-6 DO - 10.1186/s12917-017-1007-6 ID - Nardini2017 ER -