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Fig. 5 | BMC Veterinary Research

Fig. 5

From: Variation in fat content between liver lobes and comparison with histopathological scores in dairy cows with fatty liver

Fig. 5

Bright field images, comparison different morphology of fatty vacuoles and glycogen depositions of formalin fixed and snap frozen liver samples, H&E, PAS and ORO staining. a large, clear demarcated fatty vacuoles intracytoplasmatically in hepatocytes displacing the nucleus excentrically. b Intracytoplasmatical glycogen deposition (arrows) in swollen hepatocytes showing a foamy brightened cytoplasm. c ORO staining of formalin fixed tissue sample, showing clear red stained fatty vacuoles diffusely distributed in the liver lobule. d PAS staining of glycogen deposition of formalin fixed tissue samples. e ORO staining of snap frozen liver sample, diffuse red coloration of hepatocytes, unclear cell borders, sinusoids indistinct and difficult to localize. Distribution of fatty vacuoles similar to formalin fixed tissue. f PAS staining of snap frozen liver samples, diffuse pinkish coloration of hepatocytes, unclear cell borders, sinusoids indistinct and difficult to localize. Distribution of glycogen depositions similar to formalin fixed tissue

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BMC Veterinary Research

ISSN: 1746-6148

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