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Table 2 Real-time polymerase chain reaction results for the 15 selected candidate genes

From: FOXO1, PXK, PYCARD and SAMD9L are differentially expressed by fibroblast-like cells in equine synovial membrane compared to joint capsule

Target gene

Synovial membrane

Joint capsule

ATXN1

0.40 ± 0.30b

0.43 ± 0.30

COL11A1

1.35 ± 1.81

0.43 ± 0.47

COL28A1

0.36 ± 0.47b

0.17 ± 0.28

FSTL1

7.75 ± 9.99a

0.27 ± 0.32

FOXO1

3.35 ± 2.97*

0.93 ± 1.15

GALNT3

0.99 ± 1.70

0.15 ± 0.17

GRN

7.05 ± 7.63a

1.44 ± 1.66

ITPR3

1.90 ± 1.11b

1.07 ± 0.83

NF1

2.96 ± 3.42

5.60 ± 5.96

PXK

7.49 ± 7.69*

0.49 ± 0.62

PYCARD

2.70 ± 2.43*

0.34 ± 0,29

RGL2A

2.06 ± 1.70b

1.37 ± 1,66

SAMD9L

5.39 ± 4.79*

0.45 ± 0.45

SOS1

1.83 ± 2.19b

0.77 ± 0.77

TIMP3

32.26 ± 46.34a

2.95 ± 4.06

  1. Results are reported as expression of target gene relative to expression of 18S, the expression is furthermore normalised to a calibrator. The reported results are mean expression ± standard deviation. Difference between groups was tested with a Student’s t-test. *Significant difference between Synovial membrane and Joint capsule, p ≤ 0.05. aDifferential expression in synovial membrane compared to joint capsule p value ≤0.10. bTest of equal variances in the groups was not significant
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BMC Veterinary Research

ISSN: 1746-6148

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