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Table 3 Individual positive results of Egyptian serum samples from small ruminants, buffalos and camels with cELISA (ID Vet Screen Rift Valley Fever competition ELISA (multispecies), indirect immunofluorescence assay (IIFA) and virus neutralization test (VNT)

From: Seroprevalence of Rift Valley fever virus in livestock during inter-epidemic period in Egypt, 2014/15

Sample number

Herd

Age

(years)

ID vet cELISA S/N%

IIFA

VNT

ND50 value*

Result

conclusion

Sheep

 EG

12

15/OV

1

2–7

32,82

negative

negative

negative

 EG

247

15/OV

4

1–4

17,79

++

negative

positive

 EG

256

15/OV

4

1–4

16,24

negative

negative

negative

 EG

282

15/OV

4

1–4

49,48

negative

negative

negative

 EG

299

15/OV

5

1–5

not tested

+/−

negative

negative

 EG

304

15/OV

5

1–5

not tested

+/−

negative

negative

 EG

321

15/OV

5

1–5

not tested

+/−

not tested

inconclusive

 EG

328

15/OV

5

1–5

not tested

+

not tested

positive

 EG

394

15/OV

7

1–5

not tested

++

negative

inconclusive

Buffalo - small holder

 EG

4

14/BF

1

3–7

88,81

negative

10

positive

 EG

9

14/BF

1

3–7

4,90

not tested

not tested

positive

 EG

14

14/BF

1

3–7

66,06

negative

20

positive

 EG

15

14/BF

1

3–7

4,03

++

not tested

positive

 EG

20

14/BF

1

3–7

3,88

++(+)

not tested

positive

 EG

23

14/BF

1

3–7

3,65

++

not tested

positive

 EG

34

14/BF

2

3–7

4,01

++(+)

not tested

positive

 EG

38

14/BF

2

3–7

18,46

++(+)

not tested

positive

 EG

41

14/BF

3

3–7

37,71

+

not tested

positive

 EG

42

14/BF

3

3–7

10,88

++

not tested

positive

 EG

53

14/BF

4

3–7

not tested

(+)

not tested

positive

Buffalo - farms

 EG

59

15/BF

1

4–6

8,80

negative

negative

Negative

Camel - abattoir

 EG

3

14/CM

abattoir

5–7

not tested

negative

10

positive

 EG

4

14/CM

abattoir

5–7

not tested

negative

>30

positive

 EG

18

14/CM

abattoir

5–7

not tested

negative

>30

positive

 EG

32

14/CM

abattoir

5–7

5,76

++

>30

positive

 EG

46

14/CM

abattoir

5–7

not tested

negative

>30

positive

 EG

58

14/CM

abattoir

5–7

not tested

++

not tested

positive

 EG

67

14/CM

abattoir

5–7

not tested

negative

>30

positive

  1. cELISA results with a percentage of inhibition lower than 40 were defined as positive, between 40 and 50% as inconclusive and results higher than a percentage of inhibition of 50 were defined as negative. IIFA results were defined from low (+) to strong (+++) straining, or as inconclusive (+/−). ND50 values lower than 10 were defined as negative when performing VNT. Titers higher than ND50 values of 20 indicate a strong immune response
  2. Positive and inconclusive findings and results are indiciated in italics