Table 1 Primer sequences for T. circumcincta SNPs 167 and 198/200, and T. colubriformis SNP 198/200, and sequence primers for both species
Primer name | Sequence 5′-3′ | Modifications | Expected product size (pb) | Tm (°C) |
|---|---|---|---|---|
T. circumcincta | ||||
Tc SNP200 F | CACTCTTTCTGTACACCAATTG | [Btn]5 | 128 | 60 |
Tc SNP200 R | AGTGATTGAGATCGCCATAA | - | ||
Tc SNP167 F | CAAAATTCGCGAGGAGTAT | - | 276 | 60 |
Tc SNP167 R | TTCTACCAATTGGTGTACAGAAAG | [Btn]5 | ||
T. colubriformis | ||||
Tri SNP200 F | TACTTTATCAGTCCATGAGCTGG | [Btn]5 | 128 | 62 |
Tri SNP200 R | ATGGTTGAGATCTCCATAGGTTG | - | ||
Sequencing | ||||
Tc 200 Seq | AGAGCTTCATTATCGATG | - | - | 60 |
Tc 167 Seq | CGGATAGAATCATGGCT | - | - | 60 |
Tri 200 Seq | AGAGCTTCGTTATCGATGCA | - | - | 62 |