TY - JOUR AU - Esteban-Ballesteros, Myriam AU - Rojo-Vázquez, Francisco A. AU - Skuce, Philip J. AU - Melville, Lynsey AU - González-Lanza, Camino AU - Martínez-Valladares, María PY - 2017 DA - 2017/03/20 TI - Quantification of resistant alleles in the β-tubulin gene of field strains of gastrointestinal nematodes and their relation with the faecal egg count reduction test JO - BMC Veterinary Research SP - 71 VL - 13 IS - 1 AB - Benzimidazole (BZ) resistance in gastrointestinal nematodes is associated with a single nucleotide polymorphism (SNP) at codons 167, 198 and 200 in the isotype 1 of beta-tubulin gene although in some species these SNPs have also been associated with resistance to macrocyclic lactones. In the present study we compared the levels of resistance in Teladorsagia circumcincta and Trichostrongylus colubriformis by means of the faecal egg reduction test (FECRT) and the percentage of resistant alleles obtained after pyrosequencing. The study was conducted in 10 naturally infected sheep flocks. Each flock was divided into three groups: i) group treated with albendazole (ABZ); ii) group treated with ivermectin (IVM); iii) untreated group. The number of eggs excreted per gram of faeces was estimated at day 0 and 14 post-treatment. SN - 1746-6148 UR - https://doi.org/10.1186/s12917-017-0992-9 DO - 10.1186/s12917-017-0992-9 ID - Esteban-Ballesteros2017 ER -