Table 5 Virulence genes primers for PCR in this study
PCR assay | Gene | Primer sequence | PCR product size/bp |
|---|---|---|---|
Virulence- associated genes | papA | ATGGCAGTGGTGTCTTTTGGTG | 717 |
| Â | CGTCCCACCATACGTGCTCTTC | Â | |
papC | GTGGCAGTATGAGTAATGACCGTTA | 203 | |
| Â | ATATCCTTTCTGCAGGGATGCAATA | Â | |
iutA | ATCGGCTGGACATCATGGGAAC | 314 | |
| Â | CGCATTTACCGTCGGGAACGG | Â | |
vat | CTTACCTCTCTGGCACTATCTG | 1021 | |
| Â | GTCAGTGAACCGGCACC | Â | |
fimH | TGCAGAACGGATAAGCCGTGG | 508 | |
| Â | GCAGTCACCTGCCCTCCGGTA | Â | |
fyuA | TGATTAACCCCGCGACGGGAA | 787 | |
| Â | CGCAGTAGGCACGATGTTGTA | Â | |
ireA | GATGACTCAGCCACGGGTAA | 254 | |
| Â | CCAGGACTCACCTCACGAAT | Â | |
kpsMTII | GCGCATTTGCTGATACTGTTG | 272 | |
| Â | CATCCAGACGATAAGCATGAGCA | Â | |
hlyD | CTCCGGTACGTGAAAAGGAC | 904 | |
| Â | GCCCTGATTACTGAAGCCTG | Â | |
sfaS | GTCTCTCACCGGATGCCAGAATAT | 240 | |
| Â | GCATTACTTCCATCCCTGTCCTG | Â | |
hra | GTAACTCACACTGCTGTCACCT | 139 | |
| Â | CGAATCGTTGTCACGTTCAG | Â | |
cnf1 | CTTTACAATATTGACATGCTG | 446 | |
| Â | TCGTTATAAAATCAAACAGTG | Â | |
afa | TAAGGAAGTGAAGGAGCGTG | 210 | |
| Â | CCGCCCTGAAGAAGTATCAC | Â | |
kpsMTIII | TCCTCTTGCTACTATTCCCCCT | 392 | |
| Â | AGGCGTATCCATCCCTCCTAAC | Â | |
ibeA | AATGAGTGCCGCTCGTGAAGG | 548 | |
| Â | CCCCCCAGTCTCCATATTTAG | Â | |
iroN | CTCCGACGATGATAATGACGA | 440 | |
| Â | TGGGACGTTTGGTAATGATGT | Â | |
ompA | GATCAGTGCAGCACGCTGTTT | 385 | |
| Â | GGGCGAAGCAGCTCCAGTAG | Â | |
traT | CGATGAGCACAGCAATCAAGA | 486 | |
| Â | CATTATCCGTTGTCACCGTTG | Â | |
usp | GAGCGGTTATTTATTGAAATC | 448 | |
| Â | CACAGCAGTCATCAACCACTG | Â |