Table 3 List of primers used for detection of genes specificto different SPI
SPI | Primers | Sequence 5'–3' | Amplicon Size (bp) |
|---|---|---|---|
SPI1 | invAE F | CAGCGATATCCAAATGTTGC | 2168 |
invAE R | AAATGGCAGAACAGCGTCGTA | ||
hilA F | CTGCCGCAGTGTTAAGGATA | 497 | |
hilA R | CTGTCGCCTTAATCGCATGT | ||
avr F | AGACTTATATTCAGCTATCC | 1115 | |
avr R | ACATAACCCTGCTGTACCTG | ||
SPI2 | aa permease F | ACCATTCAAGAGACAATTGG | 1737 |
aa permease R | GTCCTGTTCTGGTATTACGC | ||
SPI3 | mgtC F | ATGAATCCCCAAAATTAAGG | 1153 |
mgtC R | AATCATCTGGCAAGTTAACG | ||
SPI4 | ABC Trans F | CAGTCTATCACAGCAAGGCA | 1409 |
ABC Trans R | TTATCCGGAGAACAATCACG | ||
SPI5 | pipB F | AATATTGGATGGGGGAAAAG | 230 |
pipB R | AACCTGACTCACGCAGACCT |