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Fig. 4 | BMC Veterinary Research

Fig. 4

From: Homologous prime-boost immunization with live attenuated Salmonella enterica serovar Senftenberg and its preventive efficacy against experimental challenge with various strains of S. Senftenberg

Fig. 4

The IFN-γ, IL-2 and IL-6 cytokine profiles of in vitro-stimulated PBMCs from the JOL1587-immunized and control group chickens. The relative mRNA copy numbers were determined by qRT-PCR using the comparative Ct method. I) Prime inoculation with JOL1587 at the fourth week of age and II) Prime-boost inoculation with JOL1587 at the 8 week of age. a PBS-inoculated group; b Prime immunized group and c Prime-boost immunized group. The * symbol indicates a significant increase in the cytokine levels for the JOL1587-immunized group compared to the control group chickens (P ≤ 0.05).Total six splenocytes samples were analyzed which were collected from six randomly selected chickens from group a, b and c

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