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Fig. 3 | BMC Veterinary Research

Fig. 3

From: Homologous prime-boost immunization with live attenuated Salmonella enterica serovar Senftenberg and its preventive efficacy against experimental challenge with various strains of S. Senftenberg

Fig. 3

FACS analysis for peripheral CD3 + CD4+ and CD3 + CD8+ T cell populations in the JOL1587-immunized and control group chickens. The isolated PBMCs were stained with appropriately diluted fluorescein-isothiocyanate (FITC)-labeled anti-CD3, allophycocyanin (APC)-labeled anti-CD4, and phycoerythrin (PE)-labeled anti-CD8 monoclonal antibodies. a: PBS-inoculated group; b: single immunized group and c: double immunized group. (I) Representative dot plots for CD3 + CD4+ and CD3 + CD8+ T cell populations. The population is represented as a percentage of gated cells; (II) The percentage of peripheral CD3 + CD4+ T cells; (III) The percentage of peripheral CD3 + CD8+ T cells. The values are shown as the mean ± SEM of five chickens per group. The * symbol indicates a significant increase in the percentage of peripheral T lymphocytes in the JOL1587-immunized chickens compared to the control group chickens (P ≤ 0.05)

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BMC Veterinary Research

ISSN: 1746-6148

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