Fig. 2
From: Cryptococcus gattii molecular type VGII infection associated with lung disease in a goat
Lung photomicrography. a Well-circumscribed cavitations areas of granulomatous pneumonia associated pleomorphic, yeast structures with mucinous capsule of variable thickness which show clear and refractile halo (HE stain, bar = 25 μm). b Yeast in details with oval to round, and 5–20 μm in diameter organism’s structure which capsule (carminophilic) stain in red inside of cavitations areas (cryptococcomas). (Mayer’s Mucicarmin stain, bar = 50 μm). c Cryptococcal antigen was detected by immunohistochemistry using anti-Cryptococcus polyclonal antibody, 1:50,000 diluted. Positive yeast structures stained in strong brown for Cryptococcus spp. PictureMax Kit™, DAB stain, bar = 25 μm