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Table 5 N gene based primers set used in detection of PPRV by RT-LAMP assay

From: Genetic detection of peste des petits ruminants virus under field conditions: a step forward towards disease eradication

Primer ID

Type

Sequence (5ˊ to 3ˊ)

Position* 

Length (bases)

Predicted length of amplified segment

PPRV F3

Forward outer

ACATCAACGGGTCAAAGCT

295–313

19

187 bp

PPRV B3

Backward outer

ACTCGAGGGTCCTTCAGTTG

521–502

20

PPRV/FIP

Forward inner (F1c + F2)

CCGCTGTATCAATTGCCCGGGTTTTCGGCGTGATGATCAGCATG

376–357/317–335

44

PPRV/BIP

Backward inner (B1 + B2c)

GCATCCGCCTTGTTGAGGTAGTTTTTTTGTCCAAATCAGCACCACG

400–421/481–462

46

  1. *The position of primers is indicated as per sequence position of complete genome of PPRV (Accession #hq197753)