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Table 3 Comparison of robustness of RT-LAMP assay with real-time RT-PCR for detection of PPRV in nasal swab samples collected on alternative days from experimentally infected goat

From: Genetic detection of peste des petits ruminants virus under field conditions: a step forward towards disease eradication

Day post inoculation (dpi) Detection time (Td) in RT-LAMP Real-time RT-PCR
Ct value Total time to reach Ct (minutes)a
2 39.5 36.4 80.33
4 23 31.5 76.5
6 39 34.5 78.7
8 37 34.7 78.9
12 40 34.2 78.5
  1. aTotal time includes RNA extraction + cDNA synthesis (50 min) + time to reach ct value in real-time PCR steps (50 s per cycle)