Genetic detection of peste des petits ruminants virus under field conditions: a step forward towards disease eradication

Table 3 Comparison of robustness of RT-LAMP assay with real-time RT-PCR for detection of PPRV in nasal swab samples collected on alternative days from experimentally infected goat

Day post inoculation (dpi)	Detection time (Td) in RT-LAMP	Real-time RT-PCR
Day post inoculation (dpi)	Detection time (Td) in RT-LAMP	Ct value	Total time to reach Ct (minutes)^a
2	39.5	36.4	80.33
4	23	31.5	76.5
6	39	34.5	78.7
8	37	34.7	78.9
12	40	34.2	78.5

^aTotal time includes RNA extraction + cDNA synthesis (50 min) + time to reach ct value in real-time PCR steps (50 s per cycle)

ISSN: 1746-6148