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Table 1 PPRVs isolated from clinical samples by cell culture method from four district of Punjab Province of Pakistan and detected by RT-LAMP. Time points of detection (Ct values) of cultured PPRVs in RT-LAMP assay ranged from 12 to 30 min

From: Genetic detection of peste des petits ruminants virus under field conditions: a step forward towards disease eradication

No. Type of sample Origin Place/year of outbreak Cell culture (CHS-20 cells) Td value (time point of first rise in fluorescence signal above threshold)
1 aNS Sheep DGK/2012 + 30
2 NS goat DGK/2012 + 12
3 NS goat MNW/2012 + 30
4 NS goat MNW/2012 + 10
5 NS goat MNW/2012 + 13
6 NS goat MNW/2012 + 14
7 NS goat SAH/2012 + 25
8 NS goat SAH/2012 + 20
9 NS goat SAH/2012 + 15
10 NS goat SAH/2012 + 12
11 NS goat CHK/2012 + 21
  1. +, Each isolate name includes sample ID, acronym of the district of sampling and year, DGK DG Khan, MNW Mianwali, CHK Chakwal, SAH Sahiwal. nd not determined. a NS nasal swab