Fig. 7
Limit of detection of RT-qPCR and its application for the detection of PPRV in clinical samples; Panel a. In the figure are shown amplification curves generated by serial dilution of cloned standard of PPRV N gene (463 bp fragment); Panel b. For the estimation of limit of detection, threshold cycle (Ct) values of standard dilutions from 2.06 × 106 to 2.6 × 100 were drawn against log values of copy number. Limit of detection was estimated to be ≈ 20 copies at 37.5 ± 0.9 cycle although Ct value of 40th cycle was also detectable; Panel c. Detection limit of RT-PCR used in this study was up to 2.06 × 102 copies of the plasmid; Panel d. The threshold cycle (Ct) values obtained by amplification of PPRV in clinical samples along with standard