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Fig. 4 | BMC Veterinary Research

Fig. 4

From: Genetic detection of peste des petits ruminants virus under field conditions: a step forward towards disease eradication

Fig. 4

Sensitivity of N-gene based RT-LAMP assay in terms of TCID50/ml. Fluorimetric curves in RT-LAMP assay were obtained from 10-fold Serial dilutions of PPRV with starting concentration of 10TCID50/ml; Panel a. shows amplification curves generated from these dilutions within 60-min. Panel b shows the detection of these dilutions by RT-PCR recommended by OIE amplifying 351 bp of N gene [1, 14]. The RT-LAMP was able to detect up to 10−4TCID50/ml within 60 min as compared to 10−3TCID50/ml in case of RT-PCR

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