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Table 4 Species distribution of samples in Group 2 (A). Agreement between the multiplex qPCR and the duplex PCR using the kappa index (Group 2) (B). The second panel displays the detection of B.hyodysenteriae and B.pilosicoli and the agreement between culture derived DNA vs swab derived DNA using the multiplex qPCR assay (C)

From: A novel multiplex qPCR targeting 23S rDNA for diagnosis of swine dysentery and porcine intestinal spirochaetosis

Group 2
A
Species distribution
  Culture DNA % (n)
B.hyodysenteriae 10.4% (21)
B.pilosicoli 8.9% (18)
apathogen 47% (95)
negative for Brachyspira 7.4% (15)
mixes 26.3% (53)
B.hyo + apathogen 6.5% (13)
B.pilo + apathogen 18% (37)
B.hyo + B.pilo 0.5% (1)
B.hyo + B.pilo + apathogen 1% (2)
Total samples total =202
B
Multiplex vs. Duplex on fecal culture (n = 202)
  Duplex PCR Kappa index
  + -  
Multiplex qPCR culture
B.hyodysenteriae + 34 4 0.932
- 0 164  
B. pilosicoli + 79 2 0.928
- 5 116  
C
Multiplex qPCR culture vs. swabs (n = 202)
  Culture Kappa index
  + -  
Swabs
B.hyodysenteriae + 33 4 0.853
- 5 160  
B.pilosicoli + 53 5 0.643
- 28 116