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Table 3 Result from the multiplex qPCR analysis of culture derived DNA in Group 1 (A). Agreement between the multiplex qPCR and the duplex PCR calculated with the kappa test (B). The strength of agreement was ranked accordingly: Poor (<0.00), Slight (0.00–0.20), Fair (0.21–0.40), Moderate (0,41–0.60), Substantial (0.61–0.80), Almost Perfect (0.81–1.00). B.hyo = B.hyodysenteriae; B.pilo = B.pilosicoli

From: A novel multiplex qPCR targeting 23S rDNA for diagnosis of swine dysentery and porcine intestinal spirochaetosis

Group 1
A
Species distribution
  Culture DNA % (n)
B.hyodysenteriae 19.5% (49)
B.pilosicoli 10% (25)
apathogen 36.6% (92)
mixes 33.9% (85)
B.hyo + apathogen 6.8% (17)
B.pilo + apathogen 23.1% (58)
B.hyo + B.pilo 2.4% (6)
B.hyo + B.pilo + apathogen 1.6% (4)
Total samples total =251
B
Multiplex vs. Duplex on fecal culture (n = 251)
  Duplex PCR Kappa index
  + -  
Multiplex qPCR
B.hyodysenteriae + 71 5 0.943
- 1 174  
B.pilosicoli + 92 1 0.933
- 7 151