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Table 3 Result from the multiplex qPCR analysis of culture derived DNA in Group 1 (A). Agreement between the multiplex qPCR and the duplex PCR calculated with the kappa test (B). The strength of agreement was ranked accordingly: Poor (<0.00), Slight (0.00–0.20), Fair (0.21–0.40), Moderate (0,41–0.60), Substantial (0.61–0.80), Almost Perfect (0.81–1.00). B.hyo = B.hyodysenteriae; B.pilo = B.pilosicoli

From: A novel multiplex qPCR targeting 23S rDNA for diagnosis of swine dysentery and porcine intestinal spirochaetosis

Group 1

A

Species distribution

 

Culture DNA % (n)

B.hyodysenteriae

19.5% (49)

B.pilosicoli

10% (25)

apathogen

36.6% (92)

mixes

33.9% (85)

B.hyo + apathogen

6.8% (17)

B.pilo + apathogen

23.1% (58)

B.hyo + B.pilo

2.4% (6)

B.hyo + B.pilo + apathogen

1.6% (4)

Total samples

total =251

B

Multiplex vs. Duplex on fecal culture (n = 251)

 

Duplex PCR

Kappa index

 

+

-

 

Multiplex qPCR

B.hyodysenteriae

+

71

5

0.943

-

1

174

 

B.pilosicoli

+

92

1

0.933

-

7

151