Table 2 Sensitivity and specificity calculated for the multiplex qPCR assay, compared to the gold standard duplex PCR
Method | Total samples (n) | Target spp. | Sensitivity (%) (95% CI) | Specificity (%) (95% CI) | |
|---|---|---|---|---|---|
Group 1 | multiplex qPCR | 251 | B.hyodysenteriae | 98 (86–97) | 97 (94–99) |
B.pilosicoli | 93 (93–100) | 96 (96–100) | |||
Group 2 | multiplex qPCR | 202 | B.hyodysenteriae | 100 (90–100) | 98 (94–100) |
B.pilosicoli | 94 (87–98) | 98 (94–100) | |||
swabsa | 202 | B.hyodysenteriae | 87 (72–96) | 98 (94–99) | |
B.pilosicoli | 65 (54–76) | 96 (91–99) |