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Table 2 Sensitivity and specificity calculated for the multiplex qPCR assay, compared to the gold standard duplex PCR

From: A novel multiplex qPCR targeting 23S rDNA for diagnosis of swine dysentery and porcine intestinal spirochaetosis

Method   Total samples (n) Target spp. Sensitivity (%) (95% CI) Specificity (%) (95% CI)
Group 1 multiplex qPCR 251 B.hyodysenteriae 98 (86–97) 97 (94–99)
B.pilosicoli 93 (93–100) 96 (96–100)
Group 2 multiplex qPCR 202 B.hyodysenteriae 100 (90–100) 98 (94–100)
B.pilosicoli 94 (87–98) 98 (94–100)
swabsa 202 B.hyodysenteriae 87 (72–96) 98 (94–99)
B.pilosicoli 65 (54–76) 96 (91–99)
  1. aSwab-coupled multiplex qPCR analysis compared with culture-coupled multiplex qPCR