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Table 1 Oligonucleotide primer and probe sequences with their respective reporter dye and quencher used in this study

From: A novel multiplex qPCR targeting 23S rDNA for diagnosis of swine dysentery and porcine intestinal spirochaetosis

Primer or probe name

Target

Concentration

Sequence 5′→3′

Amplicon

Multiplex qPCR

 Primer for

23S rDNA

0.4 μM

TTCGATGGAATGACACAGATTGT

128 bp

 Primer rev

23S rDNA

0.4 μM

CCGAAAGCCCAGTCACTATC

 Probe_hyo

B.hyodysenteriae

100 nM

6-FAM-CCTTAACCTTAAAGAAGCAAGCAT(BHQ-1)TTGACTCACCTCAAG-SpacerC3

 

 Probe _pilo

B.pilosicoli

100 nM

Yakima Yellow-AGGTGATGGTTATCCTCGTCGAAT-BHQ-1

 

 Probe_

apathogen

B. intermedia/

B. innocens/

B. murdochii

25 nM

Dragonfly Orange-CCTCAACCTTAAAGCAACAAGCAT(BHQ-2)TTTACTCATCACAAG-SpacerC3

 

 eGFP for

enhanced GFP

0.2 μM

GACCACTACCAGCAGAACAC

177 bp

 eGFP rev

enhanced GFP

0.2 μM

GAACTCCAGCAGGACCATG

 Probe_eGFP

enhanced GFP

25 nM

ATTO 647N-AGCACCCAGTCCGCCCTGAGCA-BHQ-3

 

Duplex PCR [12]

B.hyo_for

nox

0.5 μM

ACTAAAGATCCTGATGTATTTG

345 bp

B.hyo_rev

nox

0.5 μM

CTAATAAACGTCTGCTGC

B.pilo_for

16S rDNA

0.17 μM

AGAGGAAAGTTTTTTCGCTTC

823 bp

B.pilo_rev

16S rDNA

0.17 μM

GCACCTATGTTAAACGTCCTTG