Fig. 1

Sample groups and origin of collected samples. The 453 samples in this study were divided into two different samples groups (1–2). Group 1 consisted of cultured samples compared with the duplex PCR, Group 2 contained DNA samples directly extracted from the swabs without the culture step, and the same samples after culture of the swabs. All samples results were compared between the multiplex qPCR and the conventional duplex PCR (a). Map of the Swiss cantons from which the samples were collected. Colored cantons were included in the study and the total of B.hyodysenteriae positive samples detected with the multiplex qPCR are marked in each region (b). For 11 of the B. hyodysenteriae positive samples data about origin was not provided