Group | Immunogen | Strain for challenge | Temperature (°C) a
| Appetiteb
| Lethargyb
| Dyspneab
| Lung lesion scorec
|
---|
1 | S-8△clpP△apxIIC
| S-8 | 39.4 ± 0.2 | 0.2 ± 0.3*
| 0.2 ± 0.3**
| 0.1 ± 0.2**
| 1.4 ± 2.2*
|
2 | PBS | S-8 | 40.3 ± 0.5 | 2.4 ± 1.1 | 2.0 ± 0.6 | 2.3 ± 0.6 | 20.2 ± 11.9 |
3 | S-8△clpP△apxIIC
| K17 | 39.6 ± 0.3 | 0.3 ± 0.4*
| 0.3 ± 0.4**
| 0.4 ± 0.4**
| 1.7 ± 2.5**
|
4 | PBS | K17 | 40.5 ± 0.4 | 2.6 ± 1.7 | 2.3 ± 0.5 | 2.6 ± 0.4 | 22.8 ± 9.3 |
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a The average temperature value for each piglet during the observation period after challenge was calculated and the mean temperature value for each group was determined
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b Clinical signs were scored as described by Jolie et al [24]. Appetite was scored as follows: 0, did eat; and 1, did not eat. The total score equaled the number of 12-h periods of not eating over the 36-h observation period. Lethargy was scored as follows: 0, normal; 1, slight inactivity; 2, moderate; and 3, severe. Dyspnea was scored as follows: 0, normal; 1, slight; 2, moderate; and 3, severe. The final scores were obtained from the average of all of the data within the observation time and are expressed as arithmetic means ± SD; *, significance at a P value of <0.05; **, significance at a P value of <0.01
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c The lung lesion score was determined as described by Hannan et al [25]