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Table 2 Virulence of S-8clpPapxIIC and the wild-type strain S-8 of A. pleuropneumoniae in mice

From: Attenuated Actinobacillus pleuropneumoniae double-deletion mutant S-8∆clpP/apxIIC confers protection against homologous or heterologous strain challenge

Strains Challenge dosea (CFU) No. dead/No.tested Value of LD50b (CFU) Fold attenuationc
S-8 1.0 × 107 10/10 5.62 × 105 1
3.16 × 106 9/10
1.0 × 106 6/10
3.16 × 105 5/10
1.0 × 105 0/10
S-8clpPapxIIC 1.0 × 1010 10/10 1.12 × 109 1995
3.16 × 109 8/10
1.0 × 109 5/10
3.16 × 108 1/10
1.0 × 108 0/10
Control PBS broth 0/10 - -
  1. a Groups of ten BALB/c mice were given intraperitoneal injections of 100 μL of bacterial suspension containing various quantities of A. pleuropneumoniae strains. Survival was recorded for 14 days after infection
  2. b LD50 was calculated by the Karber’s method [23]
  3. c Fold attenuation was normalized to wild-type strain S-8