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Table 2 Virulence of S-8clpPapxIIC and the wild-type strain S-8 of A. pleuropneumoniae in mice

From: Attenuated Actinobacillus pleuropneumoniae double-deletion mutant S-8∆clpP/apxIIC confers protection against homologous or heterologous strain challenge

Strains

Challenge dosea (CFU)

No. dead/No.tested

Value of LD50b (CFU)

Fold attenuationc

S-8

1.0 × 107

10/10

5.62 × 105

1

3.16 × 106

9/10

1.0 × 106

6/10

3.16 × 105

5/10

1.0 × 105

0/10

S-8clpPapxIIC

1.0 × 1010

10/10

1.12 × 109

1995

3.16 × 109

8/10

1.0 × 109

5/10

3.16 × 108

1/10

1.0 × 108

0/10

Control

PBS broth

0/10

-

-

  1. a Groups of ten BALB/c mice were given intraperitoneal injections of 100 μL of bacterial suspension containing various quantities of A. pleuropneumoniae strains. Survival was recorded for 14 days after infection
  2. b LD50 was calculated by the Karber’s method [23]
  3. c Fold attenuation was normalized to wild-type strain S-8