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Table 2 Summarized results of real-time PCR-positive samples (FEC, combined-sedimentation-flotation, larval culture** and Ct-value)

From: Detection of Strongylus vulgaris in equine faecal samples by real-time PCR and larval culture – method comparison and occurrence assessment

Sample-No. FEC Combined-sedimentation-flotation* Larval culture Cyathostominae** Larval culture S. vulgaris ** Ct
21 20 + 16 3 27.58
144a 20 + 5 4 24.94
235 1040 +++ --- --- 33.49
318 100 + 533 0 26.90
334 900 +++ 1256 0 35.81
355 --- ++ --- --- 27.67
375 40 + 28 0 35.99
412 1720 +++ 103 0 23.40
448b 20 + 34 0 36.95
451c 20 + 57 0 35.07
460 200 ++ 535 0 35.75
497 480 +++ 1111 3 29.87
520 680 +++ 3028 1 34.97
  1. FEC = faecal egg count; Ct = cycle threshold
  2. * + = sporadic eggs; ++ = numerous; +++ = plentiful
  3. ** Number of larvae of Cyathostominae or S. vulgaris /10 g faeces
  4. --- diagnostic method not performed
  5. afollow-up examination of sample-No. 21, 2013/09/16
  6. bsample-No. 375, 2014/02/12 and
  7. csample-No: 375, 2014/02/15