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Table 2 Summarized results of real-time PCR-positive samples (FEC, combined-sedimentation-flotation, larval culture** and Ct-value)

From: Detection of Strongylus vulgaris in equine faecal samples by real-time PCR and larval culture – method comparison and occurrence assessment

Sample-No.

FEC

Combined-sedimentation-flotation*

Larval culture Cyathostominae**

Larval culture

S. vulgaris **

Ct

21

20

+

16

3

27.58

144a

20

+

5

4

24.94

235

1040

+++

---

---

33.49

318

100

+

533

0

26.90

334

900

+++

1256

0

35.81

355

---

++

---

---

27.67

375

40

+

28

0

35.99

412

1720

+++

103

0

23.40

448b

20

+

34

0

36.95

451c

20

+

57

0

35.07

460

200

++

535

0

35.75

497

480

+++

1111

3

29.87

520

680

+++

3028

1

34.97

  1. FEC = faecal egg count; Ct = cycle threshold
  2. * + = sporadic eggs; ++ = numerous; +++ = plentiful
  3. ** Number of larvae of Cyathostominae or S. vulgaris /10 g faeces
  4. --- diagnostic method not performed
  5. afollow-up examination of sample-No. 21, 2013/09/16
  6. bsample-No. 375, 2014/02/12 and
  7. csample-No: 375, 2014/02/15