Lipopolysaccharide derived from the digestive tract triggers an inflammatory response in the uterus of mid-lactating dairy cows during SARA

Table 2 The list of primer sequences used for amplification of qRT-PCR

Gene	Forward primers	Reverse primers	Accession number	Product size (bp)
TLR-4	GGACCCTTGCGTACAGGTTG	GGAAGCTGGAGAAGTTATGGC	NM_174198.6	244
TRAF-6	GCGGCCTTCAAGTTAGGAGA	TCATCAACTGCTCGTTCGGG	NM_001034661.2	141
NF-κB	ACGATCGTCACCGGATTGAG	GGTGCTGAGAGATGGCGTAA	XM_005699996.1	194
β-actin	CTCTTCCAGCCTTCCTTCCT	GGGCAGTGATCTCTTTCTGC	AY141970	178
IL-6	GGAGGAAAAGGACGGATGCT	GGTCAGTGTTTGTGGCTGGA	EU276071.1	226
IL-8	CCTCTTGTTCAATATGACTTCCA	GGCCCACTCTCAATAACTCTC	NM_173925.2	170
TNF-α	GCTCTTACCGGAACACTTCG	GGACACCTTGACCTCCTGAA	NM_173966	238
IL-1β	AACCGAGAAGTGGTGTTCTGC	TTGGGGTAGACTTTGGGGTCT	NM_174093	167
MyD88	AAAGCCCGAGTGTTTTGATG	TCACATTCCTTGCTTTGCAG	NM_001014382	234
LBP	GCAAGATCACTGGATTCTTGGA	AAAACAGGAAGTCCTTGTGGATC	NM_001038674.2	228

ISSN: 1746-6148