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Table 1 Mortality, mean death time, 50% infectious dose, 50% lethal dose and number of birds shedding for three H5N2 highly pathogenic avian influenza viruses in 4-week old directly inoculated and contact exposed broad-breasted white turkeys

From: H5N2 Highly Pathogenic Avian Influenza Viruses from the US 2014-2015 outbreak have an unusually long pre-clinical period in turkeys

Isolate

Dose (EID50/bird)

Mortality

Mean death time (days)

Number of turkeys shedding

Seroconversion

50% Turkey infectious dosee

Inoculated

Contact exposed

Inoculated

Contact exposed

Inoculated

Contact exposed

Inoculated

Contact exposed

A/northern pintail/WA/40964/2014

102

0/5a

0/3a

NAb

NA

0/5d

0/3d

0/5i

0/3i

105 EID50 f

104

0/5

0/3

NA

NA

0/5

0/3

0/5

0/3

106

5/5

3/3

5.3 (4–7)gh

7.6 (7–8)cg

5/5

3/3

NA

NA

106

16/16

NA

NA

16/16

NA

NA

NA

A/turkey/MN/12582/2015

102

0/5

0/3

NA

NA

0/5

0/3

0/5

0/3

105 EID50

104

0/5

0/3

NA

NA

0/5

0/3

0/5

0/3

106

5/5

3/3

5.9 (3–10)

8.0 (6–10)

5/5

3/3

NA

NA

106

16/16

NA

NA

16/16

NA

NA

NA

A/chicken/IA/13388/2015

102

0/5

0/3

NA

NA

0/5

0/3

0/5

0/3

103 EID50

104

5/5

3/3

5.4 (1–7)

9.0 (6–10)

3/5

3/3

NA

NA

106

5/5

3/3

5.6 (3–13)

7.6 (2–13)

5/5

3/3

NA

NA

106

16/16

NA

NA

16/16

NA

NA

NA

  1. aNumber dead/total
  2. b NA not applicable. Either no mortality or no birds survived for antibody testing
  3. cCalculated from the day of placement with the inoculated turkeys
  4. dNumber shedding/total
  5. e50% infectious dose was equal to the 50% lethal dose for all isolates
  6. fEID50 = 50% egg infectious dose
  7. gMean death time days (range of mortality in days). For inoculated birds includes data from all 21 turkeys exposed to 106 EID50/bird
  8. hMean death times are combined for directly exposed turkeys in the same dose group for each isolate
  9. iNumber seroconverted/total