Biochemical and proteomic analyses of the physiological response induced by individual housing in gilts provide new potential stress markers

Table 4 Selected differentially expressed proteins at D3 and D5 versus D1 (basal levels) in the H and HS groups identified by proteomics (DIGE or iTRAQ)

Protein	Technique	Day 3		Day 5
Protein	Technique	FC H	FC HS	FC H	FC HS
Immune system
Alpha-1- antichymotrypsin 3	DIGE	1.74	1.20	1.36	1.16
Galectin-1	iTRAQ	1.64	2.31	1.08	2.56
Haptoglobin (spot 1)	DIGE	3.24	2.19	1.71	1.63
Haptoglobin (spot 2)	DIGE	1.48	1.73	1.29	1.34
Lactotransferrin	iTRAQ	1.21	1.38	1.54	1.08
Protegrin-3	iTRAQ	1.45	1.75	1.46	1.69
Transport
Apolipoprotein A-I	DIGE	−1.38	−1.70	−1.26	−1.53
Apolipoprotein A-I	iTRAQ	−1.28	−1.62	−1.04	−1.64
Apolipoprotein E	iTRAQ	−1.89	−1.96	−1.22	−1.60
Apolipoprotein C-III	iTRAQ	−1.56	−1.79	−1.18	−1.72
Apolipoprotein R	iTRAQ	−1.36	−1.78	−1.03	−1.73
Apolipoprotein M	iTRAQ	−1.49	−1.80	−1.26	−2.02
Antioxidant defenses
Glutathione peroxidase 3	iTRAQ	−1.45	−1.58	−1.06	−1.51
Peroxiredoxin 2	DIGE	3.73	5.78	2.09	4.86
Peroxiredoxin 2	iTRAQ	1.99	2.31	1.80	2.17

Increased proteins at D3 are represented as positive fold-changes (FC) and decreased proteins as negative FC. Proteins are classified according to their biological function. The complete list of differentially expressed proteins identified by MS is shown in Additional file 1 (DIGE) and Additional file 2 (iTRAQ)

ISSN: 1746-6148