Fig. 6

mRNA expression levels of the TLRs and MDA5 in the magnum and uterus of laying hens infected with NDV. Total RNA was extracted from the infected and control groups (n = 3 per group). The TLRs and MDA5 were quantified using appropriate primers specific to the chicken and SYBR Green-based real-time (RT) PCR. The data represent the mean ± standard error of the mean (SEM) for three chickens. Panels a, b, c, and d depict the results for TLR3, TLR7, TLR21, and MDA5, respectively, in the magnum and uterus. Statistical analysis was performed by comparison with the healthy chickens. *P <0.05, **P <0.01, ***P <0.001