A proteomic approach to the development of DIVA ELISA distinguishing pigs infected with Salmonella Typhimurium and pigs vaccinated with a Salmonella Typhimurium-based inactivated vaccine

Table 1 Primer pairs of DNA sequences for used proteins (or polypeptide fragments) and their expected molecular weight including HisTag

Protein	Forward primer 5′-3′	Reverse primer 5′-3′	Expected protein molecular weight
BamB	ATGCAATTGCGTAAATTACTTCTGCC	TTAACGCGTAATCGCGTAGACC	45.8 kDa
FliC-frag	ACGCTGAATGTGCAACAAAAATATAAGGTC	ACCTTCGGCTTTACTTGCAGCG	25.8 kDa
MetQ	ATGGCGTTCAAATTCAAAACCTTTGCGG	TTACCAGCCTTTCACCGCGCC	33.3 kDa
PrgI	ATGGCAACACCTTGGTCAGGCTATC	TTAACGGAAGTTCTGAATAATGGCAGCATC	12.7 kDa
SipC	ATGTTAATTAGTAATGTGGGAATAAATCCCGCC	TTAAGCGCGAATATTGCCTGCGAT	46.8 kDa
SipD	ATGCTTAATATTCAAAATTATTCCGCT	TTATCCTTGCAGGAAGCTTTT	41.0 kDa
SseB	ATGTCTTCAGGAAACATCTTATGGGGAA	TCATGAGTACGTTTTCTGCGCTATC	25.4 kDa
SurA	ATGAAGAACTGGAAAACGCTGCTTC	TTAGTTACTCAAAATCTTAACGTAAGCGCTG	51.1 kDa
SipB	ATGGTAAATGACGCAAGTAGCATTAGCCG	TTATGCGCGACTCTGGCGCAGAATAAAAC	66.3 kDa
OppA	ATGTCTAACATCACGAAAAAAAGTTTG	TTAATGTTTGATAATATATAAGTTTTTCACATAAAT	65.1 kDa

ISSN: 1746-6148