Fig. 4
From: Bovine lactoferrin free of lipopolysaccharide can induce a proinflammatory response of macrophages
Presence of phosphorylated form of IκB in HEK cell line expressing no TLR (left, HEK0) or the same cell line expressing TLR4a-MD2-CD14 (right, HEK4a) after stimulation with (from left o right) 1 μg/ml of lipopolysaccharide (LPS); 100 μg/ml of unpurified (LF + LPS) or purified lactoferrin (LF pur) containing 4.10 EU/ml or 0.05 EU/ml, respectively. Cells cultivated in LPS-free medium were used as a control. β-actin served as loading control