Fig. 3
From: Bovine lactoferrin free of lipopolysaccharide can induce a proinflammatory response of macrophages
Expression of mRNA for IL8 (a) and TNFα (b) by HEK cell line expressing TLR4a-MD2-CD14 after stimulation with 1 μg/ml of lipopolysaccharide (LPS); unpurified lactoferrin (LF unpur) and purified lactoferrin (LF pur) containing 4.10 EU/ml and 0.05 EU/ml, respectively. Cells stimulated with unpurified LF in combination with polymyxine B are marked as LF-PMB. Cells cultivated in LPS-free medium were used. Data are shown as mean ± SD of 5 repetitions. Different letters show statistical significance when compared with control (p < 0.05)