Fig. 2
From: Recombinase polymerase amplification assay for rapid detection of lumpy skin disease virus
The results layout of one LSDV RPA assay run. Fluorescence development over time using a dilution range of 107–101 molecules/μl of the DNA molecular standard (Graph generated by ESEquant tubescanner software). 107 represented by black line; 106, gray; 105, red; 104, blue; 103, green; 102, cyan; 101, dark khaki; negative control, orange. The LSDV RPA assay detected down to 10 DNA molecules/reaction. After 230 s, the strip was taken out of the tubescanner for mixing, therefore, no fluorescence signals were recorded