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Table 2 Salivary levels of leptin (median, 25th and 75th percentiles; ng/mL) in pigs submitted to different models. A stress model, where samples were taken to 7 pigs before (baseline) and immediately (T0), 15 min (T15) and 30 min (T30) after immobilization with nose-snare. A inflammation model, were samples were taken on 5 pigs before (baseline) and after turpentine injection at 24 h (T24h), 48 h (T48h), 72 h (T72) and 7 days (T7d). A fasting model, where samples were taken to 20 pigs 15 min after of 24 h of fasting and before feeding the animals (T1), immediately (T2) and 90 min (T3) after the food administration. Finally, a growing model where samples were taken to 6 pigs at 60, 90, and 150 days of age

From: Development and validation of an assay for measurement of leptin in pig saliva

Stress model Inflammation model Fasting model Growing model
n = 7 n = 5 n = 20 n = 6
Time of sampling Median (25–75th) Time of sampling Median (25–75th) Time of sampling Median (25–75th) Time of sampling Median (25–75th)
Baseline 32.1 (16–90) Baseline 28.6 (24–36) T1 25.7† (21–36) 60 days 21.9 (19–37)
T0 19.1 (13–72) T24h 72.3* (55–156) T2 117.8* (79–255) 90 days 36.1 (27–83)
T15 16.6 (12–29) T48h 36.0 (30–90) T3 24.1† (16–41) 150 days 42.8* (36–84)
T30 13.6 (8–56) T72h 40.3 (37–151)     
   T7d 31.6† (24–33)     
  1. * Significant (P < 0.05) difference with the first sampling (Baseline, T1 and 60 days, for inflammation, fasting and growing models respectively)
  2. † Significant (P < 0.05) difference compared to second sampling (T24h for inflammation model and T2 for fasting model)