Fig. 1
From: Development and validation of an assay for measurement of leptin in pig saliva
a SDS-PAGE of the rabbit polyclonal antibody against the recombinant porcine leptin. Lane 1: molecular weight markers (Invitrogen, Barcelona, Spain). Lane 2: purified immunoglobulin G (5 μg). b Western blotting of salivary leptin. Lane 1: molecular mass markers in kDa (Invitrogen, Barcelona, Spain). Lane 2: recombinant pig leptin. Lanes 3: unconcentrated pig saliva samples. Lane 4: 10-fold concentrated pig saliva sample